Application of Simple Western to analysis of antibody target affinity and specificity

Accurate characterization of monoclonal antibody (mAb) target affinity and specificity is critical for antibody development, validation, and diagnostic applications. The Simple Western™ platforms perform capillary–based immunoassays, are fully automated and provide highly reproducible and reliable quantitation. In this webinar, we demonstrate the use of Simple Western platforms to quantify expression levels of mAb in various cell lines, validate antibody specificity using peptide inhibition assays with results comparable to immunohistochemistry data, estimate and compare mAb clone binding affinities, assess effects of substitution of amino acid residues in mAb epitopes and identify and quantify off-target effects. Simple Western technology offers robust, protein analysis that can accelerate selection and optimization of antibody candidates for further therapeutic development and perform highly precise quantitation of biomarkers.

What you will learn:

An overview of Simple Western technology and how it enables quantitative peptide inhibition and dose-response workflows/assays to estimate mAb target affinity.
Approaches for defining mAb epitopes using peptide truncation and alanine scanning strategies
Methods for identifying and quantitatively assessing off‑target antibody binding using Simple Western–based workflows

First name

Last name

Company

Job Title

Country

ZIP/Postal Code

Organization Type

Would you like to connect with a technical expert from our sponsor Bio-Techne?

No cost to register, subject to confirmation

By registering for this event, you accept that you may receive direct communication from Endpoints News and/or the sponsor(s). View how we use your data here.

If you are experiencing problems with your registration, please try the Zoom registration page.

Mark Dodson

Staff Scientist, Roche Tissue Diagnostics

Originally from Fresno, California, Mark Dodson earned his BS and MA in Microbiology from California State University and his PhD in Molecular Biology from the University of California, Berkeley. His academic career included postdoctoral fellowships at Sloan Kettering Cancer Center and the Beckman Center for Molecular and Genetic Medicine at Stanford University. Notably, his work at Stanford on the Herpes Simplex Virus Type1 helicaseprimase contributed to the development of the antiherpetic drugs amenamevir and pritelivir.

Following his training, Dr. Dodson served as an Assistant Professor of Biochemistry at the University of Arizona College of Medicine, where he taught and supervised research on herpesvirus DNA replication. He later transitioned to the pharmaceutical industry, conducting drug discovery research at Sanofi before joining Roche Tissue Diagnostics, where he is currently a Staff Scientist focused on developing oncology immunohistochemical diagnostics.

Madhura Nayak

Senior Product Manager, Bio-Techne

Madhura Nayak is a Senior Product Manager for Bio-Techne’s Simple Western solutions. Previously, Madhura worked across the biotech and life sciences industry supporting product strategy, scientific applications, and customer workflows in protein analysis, spatial biology, and cell therapy markets. She has an undergraduate degree in Bioengineering and earned her Master from the University of Pennsylvania with a concentration in Biopharmaceuticals and Engineering Biotechnology.

moderator

Jaime Green

Freelance Journalist & Science Writer

Jaime Green is a freelance science writer and author. Her work has appeared in The New York Times Magazine, The Atlantic, Aeon, Popular Science, Slate, Astrobites, and elsewhere. She is the series editor of The Best American Science and Nature Writing and author of The Possibility of Life: Science, Imagination, and Our Quest for Kinship in the Cosmos.