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Leveraging acoustic focusing for fast and precise high-parameter flow cytometry panels
The advent of spectral data unmixing has significantly enhanced the capability for high parameter multicolor analysis in flow cytometry. However, this advancement has also led to increased acquisition and analysis times for high-dimensional data. To address these challenges, the Invitrogen™ Attune™ Xenith™ Flow Cytometer has been developed, incorporating proven acoustic focusing core technology to deliver efficient, rapid, and accurate full-spectrum data acquisition.
In this study, we utilized a 37-color mouse panel to perform comprehensive immunophenotyping of tissue digests. Our results demonstrate that the Attune Xenith Flow Cytometer can acquire data at reduced instrument run times without excessive sample dilution, even with clog-prone samples. At acquisition rates of 1,000 µL/minute, data resolution was maintained, and we identified distinct subpopulations, including rare subsets, from spleen and bone marrow.
The high sample throughput capability of this platform significantly reduces time to results while maintaining uncompromised spectral resolution, even with highly complex panels. This efficiency helps facilitate the identification of rare cell subsets from tissue samples and enhances overall laboratory productivity.
